Discussions

Several techniques are available to evaluate the quality, quantity, and function of the therapeutic non-IgG antibodies produced, such as enzyme-linked immunosorbent assay (ELISA), flow cytometry, immunoblotting, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Besides, our innovative protein analytical methods can provide you with important and detailed information about IgA, IgE, and IgM therapeutic antibodies and help you screen throughout the development phase.

Technology for Non-IgG Antibody Characterization at Creative Biolabs
After gaining high-quality non-IgG antibodies by our production and purification, we provide a series of strategic approaches with detailed characterization of your antibody, including but not limited to:

Antibody titration and quantization by ELISA
High-performance liquid chromatography (HPLC) analysis
Intact mass determination by mass spectrometry of heavy and light chains
N- and C-terminal sequencing of the heavy and light chain by Edman degradation and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS)
Western blot (WB) assay
SDS-PAGE assay
Epitope binning
Affinity ranking
EC50 and IC50
Cross-reactivity
Cell-based screening